دانش و فناوری هوافضا

دانش و فناوری هوافضا

منداب و امنیت غذایی در محیط‌های فراسیاره‌ای

نوع مقاله : مقاله پژوهشی

نویسنده
گروه فیزیولوژی هوافضایی، پژوهشگاه هوافضا، وزارت علوم، تحقیقات و فناوری، تهران، ایران
چکیده
گیاه راکت یا منداب (Eruca sativa) توسط سازمان ناسا برای پرورش در فضای خارج از جو و کشت روی سطح مریخ انتخاب شده است. در پژوهش حاضر، تاثیر محیط خلا شبیه سازی شده فضا بر شاخص جوانه زنی، محتوای پروتئینی، فنل و فلاونوئید کل و فعالیت آنتی اکسیدانی بذر خشک گیاه راکت مورد مطالعه قرار گرفت. به منظور شبیه سازی شرایط خلا فضا، نمونه های بذر راکت درون محفظه شبیه ساز خلا سامانه های فضایی قرار گرفتند. نتایج افزایش معنادار شاخص جوانه زنی بذر را در بازه زمانی 5 روز در گروه تحت تیمار خلا نشان داد. محتوای فنل کل در بذرهای تحت تیمار خلا در مقایسه با بذرهای گروه کنترل افزایش معناداری را از لحاظ آماری نشان داد. بذرهای تحت تیمار خلا محتوای پروتئین کل بالاتری را نسبت به بذرهای گروه شاهد داشتند. نیمرخ پروتئینی بذر در گروه های کنترل و خلا، 10 باند پروتئینی مشخص را در محدوده وزن مولکولی 4/47 تا 67/46 کیلودالتون نشان داد که شدت باندها بین دو گروه متفاوت بود. افزایش محتوای پروتئینی کل و افزایش شدت باندهای پروتئینی در وضعیت خشک امر نادری می باشد که در مطالعه حاضر مشاهده شد. نتایج حاصل از مطالعه حاضر می تواند کاربردهای مهمی در زمینه امنیت غذایی و ذخیره بذر در بانک های بذر سیاره زمین و همچنین محیط های فراسیاره ای داشته باشد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Eruca and food security in extra-planetary environments

نویسنده English

فاطمه موسوی
Air and Space Physiology Research Group, Aerospace Research Institute (ARI), Ministry of Science, Research and Technology (MSRT), Tehran
چکیده English

Rocket (Eruca sativa) has been selected by NASA for growing in outer space and farming on Mars. The present study studied the effect of the simulated vacuum environment on germination index, protein content, total phenol and flavonoid and antioxidant activity of dry rocket seed was studied. In order to simulate the vacuum conditions of space, the dry rocket seeds were placed in the vacuum simulator chamber of space systems. The results showed a significant increase in the seed germination index in the 5-day interval in the group under vacuum treatment. The content of total phenol in the seeds under vacuum treatment showed a statistically significant increase compared to the seeds of the control group. The seeds under vacuum treatment had a higher total protein content than the seeds of the control group. The seed protein profile in the control and vacuum groups showed 10 distinct protein bands in the molecular weight range of 4.47 to 67.46 kilodaltons, and the intensity of the bands was different between the two groups. The increase in total protein content and the intensity of protein bands in the dry state is rare as was observed in the present study. The results of the present study can have important applications in the field of food security and seed storage in seed banks of the Earth as well as extraterrestrial environments.

کلیدواژه‌ها English

vacuum
simulator
seed
mars
outer space
Eruca
rocket
[1] M. Wang, K. Danz, V. Ly, and M. Rojas-Pierce, Microgravity enhances the phenotype of Arabidopsis zigzag-1 and reduces the Wortmannin-induced vacuole fusion in root cells, npj Microgravity, vol. 8, no. 1, p. 38, 2022.
[2] G. Clément and K. Slenzka, Fundamentals of space biology: research on cells, animals, and plants in space. Springer Science & Business Media, 2006.
[3] F. Mousavi, History of Plant Exploration Scientific Missions: Goals and Technologies, Journal of Technology in Aerospace Engineering, vol. 5, no. 2, pp. 1-9, 2021.
[4] F. Mousavi, History of Plant Exploration Scientific Missions: Goals and Technologies, Technology in Aerospace Engineering, vol. 5, no. 2, pp. 1-9, 2021.
[5] J. P. Vandenbrink and J. Z. Kiss, Space, the final frontier: A critical review of recent experiments performed in microgravity, Plant Science, vol. 243, pp. 115-119, 2016.
[6] A. L. Paul, R. M. Wheeler, H. G. Levine, and R. J. Ferl, Fundamental plant biology enabled by the space shuttle, American Journal of botany, vol. 100, no. 1, pp. 226-234, 2013.
[7] F. Mousavi, Plant germplasm and extreme conditions of outer space, Space Science and Technology, vol. 16, no. English Special Issue, pp. 65-71, 2023.
[8] F. Mousavi, The effect of extreme temperature fluctuations simulated of space on the electrophoretic profile of tomato (Lycopersicum esculentum Mill.) seed storage proteins, Space Science and Technology, vol. 16, no. 4, pp. 83-89, 2023.
[9] K. Weitbrecht, K. Müller, and G. Leubner-Metzger, First off the mark: early seed germination, Journal of experimental botany, vol. 62, no. 10, pp. 3289-3309, 2011.
[10] M. Nagel and A. Börner, The longevity of crop seeds stored under ambient conditions, Seed Science Research, vol. 20, no. 1, pp. 1-12, 2010.
[11] N. Sano, L. Rajjou, H. M. North, I. Debeaujon, A. Marion-Poll, and M. Seo, Staying alive: molecular aspects of seed longevity, Plant and Cell Physiology, vol. 57, no. 4, pp. 660-674, 2016.
[12] A. Kivilaan and R. S. Bandurski, The one hundred‐year period for Dr. Beal's seed viability experiment, American Journal of Botany, vol. 68, no. 9, pp. 1290-1292, 1981.
[13] C. Walters, L. M. Wheeler, and J. M. Grotenhuis, Longevity of seeds stored in a genebank: species characteristics, Seed Science Research, vol. 15, no. 1, pp. 1-20, 2005.
[14] A. Steiner and P. Ruckenbauer, Germination of 110-year-old cereal and weed seeds, the Vienna Sample of 1877. Verification of effective ultra-dry storage at ambient temperature, Seed Science Research, vol. 5, no. 4, pp. 195-199, 1995.
[15] J. Shen‐Miller, M. B. Mudgett, J. W. Schopf, S. Clarke, and R. Berger, Exceptional seed longevity and robust growth: ancient sacred lotus from China, American Journal of Botany, vol. 82, no. 11, pp. 1367-1380, 1995.
[16] A. M. Visscher, C. E. Seal, R. J. Newton, A. L. Frances, and H. W. Pritchard, Dry seeds and environmental extremes: consequences for seed lifespan and germination, Functional Plant Biology, vol. 43, no. 7, pp. 656-668, 2016.
[17] M. B. Fleming, L. M. Hill, and C. Walters, The kinetics of ageing in dry-stored seeds: a comparison of viability loss and RNA degradation in unique legacy seed collections, Annals of Botany, vol. 123, no. 7, pp. 1133-1146, 2019.
[18] X. Xin, X. H. Lin, Y. C. Zhou, X. L. Chen, X. Liu, and X. X. Lu, Proteome analysis of maize seeds: the effect of artificial ageing, Physiologia Plantarum, vol. 143, no. 2, pp. 126-138, 2011.
[19] E. Mallah et al., The influence of Eruca sativa (Arugula) on pharmacokinetics of sildenafil in rats, Neuroendocrinology Letters, vol. 38, no. 4, pp. 295-300, 2017.
[20] H. Hniličková, F. Hnilička, J. Martinkova, and K. Kraus, "Effects of salt stress on water status, photosynthesis and chlorophyll fluorescence of rocket, Plant, Soil and Environment, vol. 63, no. 8, pp. 362-367, 2017.
[21] J. O. Chandler et al., Rocket science: The effect of spaceflight on germination physiology, ageing, and transcriptome of Eruca sativa seeds, Life, vol. 10, no. 4, p. 49, 2020.
[22] S. Baliyan et al., Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa, Molecules, vol. 27, no. 4, p. 1326, 2022.
[23] M. H. Sin, Total phenolic content and anti-oxidant potential of Ficus deltoidea using green and non-green solvents, Journal of Pharmaceutical Negative Results, vol. 8, no. 1, pp. 15-19, 2017.
[24] A. Djeridane, M. Yousfi, B. Nadjemi, D. Boutassouna, P. Stocker, and N. Vidal, Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds, Food chemistry, vol. 97, no. 4, pp. 654-660, 2006.
[25] S. Shinwari, F. Akbar, M. A. Rabbani, A. S. Mumtaz, and Z. K. Shinwari, Evaluation of genetic diversity in different genotypes of Eruca sativa from Pakistan by SDS-PAGE analysis, Pakistan Journal of Botany, vol. 45, no. 4, pp. 1235-1240, 2013.
[26] M. Bradford, A rapid and sensitive method for the quantities of microgram quantities of protein utilizing the principle of protein dye intetaction, Anal biochem, vol. 72, pp. 248-254, 1976.
[27] L. Rajjou et al., Seed germination and vigor, Annual review of plant biology, vol. 63, pp. 507-533, 2012.
[28] F. J. Villalobos, F. Orgaz, and E. Fereres, Sowing and planting, Principles of Agronomy for Sustainable Agriculture, pp. 217-227, 2016.
[29] P.-Y. Lin and H.-M. Lai, Bioactive compounds in legumes and their germinated products, Journal of agricultural and food chemistry, vol. 54, no. 11, pp. 3807-3814, 2006.
[30] S. Aharon et al., Effects of baking, roasting and frying on total polyphenols and antioxidant activity in colored chickpea seeds, Food and Nutrition Sciences, vol. 2012, 2012.
[31] O. Monje, G. Stutte, G. D. Goins, D. Porterfield, and G. Bingham, Farming in space: environmental and biophysical concerns, Advances in Space Research, vol. 31, no. 1, pp. 151-167, 2003.
[32] M. C. Kyriacou et al., Micro-scale vegetable production and the rise of microgreens, Trends in food science & technology, vol. 57, pp. 103-115, 2016.
[33] V. De Micco, C. Amitrano, P. Vitaglione, R. Ferracane, M. Pugliese, and C. Arena, Effect of light quality and ionising radiation on morphological and nutraceutical traits of sprouts for astronauts’ diet, Acta Astronautica, vol. 185, pp. 188-197, 2021.
[34] S. Pukacka and E. Ratajczak, Age-related biochemical changes during storage of beech (Fagus sylvatica L.) seeds, Seed Science Research, vol. 17, no. 1, pp. 45-53, 2007.
[35] F. Boughalleb, M. Mahmoudi, R. Abdellaoui, B. Yahia, S. Zaidi, and N. Nasri, Effect of long‐term storage on phenolic composition, antioxidant capacity, and protein profiles of Calicotome villosa subsp. intermedia seeds, Journal of food biochemistry, vol. 44, no. 1, p. e13093, 2020.
[36] R. Abdellaoui, A. Souid, D. Zayoud, and M. Neffati, Effects of natural long storage duration on seed germination characteristics of Periploca angustifolia Labill, African Journal of Biotechnology, vol. 12, no. 15, 2013.
[37] E. Businge, J. Bygdell, G. Wingsle, T. Moritz, and U. Egertsdotter, The effect of carbohydrates and osmoticum on storage reserve accumulation and germination of Norway spruce somatic embryos, Physiologia plantarum, vol. 149, no. 2, pp. 273-285, 2013.
[38] M. Nigam et al., Accelerated ageing induces physiological and biochemical changes in tomato seeds involving MAPK pathways, Scientia Horticulturae, vol. 248, pp. 20-28, 2019.
[39] A. Jain, Seed Storage Protein, Functional Diversity and Association with Allergy, Allergies, vol. 3, no. 1, pp. 25-38, 2023.
[40] G. P. Kaushal, J. S. Sital, and I. S. Bhatia, Studies on Taramira seed (Eruca sativa Lam.) proteins, Journal of Agricultural and Food Chemistry, vol. 30, no. 3, pp. 431-435, 1982.
[41] T. S. Templeman, A. E. DeMaggio, and D. A. Stetler, Biochemistry of fern spore germination: Globulin storage proteins in Matteuccia struthiopteris L, Plant physiology, vol. 85, no. 2, pp. 343-349, 1987.
[42] R. E. Aluko, T. McIntosh, and M. Reaney, Comparative study of the emulsifying and foaming properties of defatted coriander (Coriandrum sativum) seed flour and protein concentrate, Food research international, vol. 34, no. 8, pp. 733-738, 2001.
[43] F. ALSalman, Isolation and molecular and nutritional properties of proteins from Eruca sativa leaves and seeds. McGill University (Canada), 2016.
[44] F. Mousavi, Y. Shahali, Z. Pourpak, A. Majd, and F. Ghahremaninejad, Year-to-year variation of the elemental and allergenic contents of Ailanthus altissima pollen grains: an allergomic study, Environmental monitoring and assessment, vol. 191, pp. 1-10, 2019.
[45] F. Mousavi, A. Majd, Y. Shahali, F. Ghahremaninejad, R. S. Shoormasti, and Z. Pourpak, Immunoproteomics of tree of heaven (Ailanthus atltissima) pollen allergens,  Journal of proteomics, vol. 154, pp. 94-101, 2017.
[46] R. H. Sammour, Effect of ageing on the major reserve molecules and their related enzyme in natural aged seeds of flax, Journal of Islamic Academy of Sciences, vol. 2, no. 4, pp. 247-251, 1989.
[47] P. Coello and J. M. Vázquez-Ramos, "Maize DNA polymerase 2 (an α-type enzyme) suffers major damage after seed deterioration, Seed Science Research, vol. 6, no. 1, pp. 1-7, 1996.
[48] K. Vishwanath, K. Prasanna, R. Gowda, S. R. Prasad, S. Narayanaswammy, and H. Pallavi, "Influence of accelerated ageing on total soluble seed protein profiles of tomato, SEED RESEARCH-NEW DELHI-, vol. 35, no. 2, p. 194, 2007.